statistical software prism v.5.00 Search Results


spectra manager v 500 software  (JASCO Inc)
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JASCO Inc spectra manager v 500 software
Spectra Manager V 500 Software, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prism v.5.00 software package  (GraphPad Software Inc)
90
GraphPad Software Inc prism v.5.00 software package
Prism V.5.00 Software Package, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ter119-v500  (Becton Dickinson)
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v500-cd90.2  (Becton Dickinson)
90
Becton Dickinson v500-cd90.2
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
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network v.5.0.0.3  (FLUXUS ENGINEERING LIMITED)
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FLUXUS ENGINEERING LIMITED network v.5.0.0.3
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
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klinik akut v 500 disinfection unit  (Viromed Inc)
90
Viromed Inc klinik akut v 500 disinfection unit
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
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v500-conjugated streptavidin  (Becton Dickinson)
90
Becton Dickinson v500-conjugated streptavidin
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
V500 Conjugated Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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image processor sigmascan pro v 5.0.0  (SYSTAT)
90
SYSTAT image processor sigmascan pro v 5.0.0
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
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visual eng infrared goggles micromedical v5.00  (Micromedical Technologies Inc)
90
Micromedical Technologies Inc visual eng infrared goggles micromedical v5.00
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
Visual Eng Infrared Goggles Micromedical V5.00, supplied by Micromedical Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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graphpad prism v.5.00  (GraphPad Software Inc)
90
GraphPad Software Inc graphpad prism v.5.00
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
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v 500  (Bio-Rad)
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Bio-Rad v 500
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
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cd16 mouse anti-human, v500  (Fisher Scientific)
90
Fisher Scientific cd16 mouse anti-human, v500
a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - <t>Thy1</t> + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).
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Image Search Results


a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - Thy1 + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).

Journal: bioRxiv

Article Title: Remodeling of Il4-Il13-Il5 locus underlies selective gene expression

doi: 10.1101/2022.07.22.501024

Figure Lengend Snippet: a , REs identified in this study were selectively deleted (red bars) to generate 6 mouse strains, and subjected to papain induced lung inflammation ( b ) to compare their type 2 response. WT (naïve; n=24, papain; n=37), KHS-KO (naïve; n=8, papain; n=11), 5HS-I(a+b+c+d)-KO (naïve; n=4, papain; n=10), 5HS-Ie KO (naïve; n=12, papain; n=8), 5HS-II KO (naïve; n=14, papain; n=11), and 5HS-III(a+b+c) KO (naïve; n=11, papain; n=16) were analyzed in two to five independent experiments. c , PAS staining of the lung from mice treated with papain. A representative image from each genotype is shown. d , Histological scores were calculated for each genotype group and compared with statistical evaluation (asterisks). e , Cell numbers recovered from the lungs were compared among 6 genotypes (color code as in d) in naïve and papain challenged conditions. Cells evaluated were eosinophils (CD11b + Siglec-F + ), neutrophils (CD11b + Gr1 + ), Th2 (CD3ε + TCRβ + CD4 + CD44 + Foxp3 - GATA3 + ) and ILC2s (Lin - Thy1 + CD127 + GATA3 + ). f , Cytokine production from ILC2s (top panels) and Th2 (bottom panels) were evaluated by FACS for IL-4, IL-13 and IL-5. g , Graphical summary of impact on cytokine production due to RE deletion in mice. Cell type dependent differential impact was noted for some REs. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 (Student’s t test).

Article Snippet: The following antibodies were used for flow cytometry and cell purification in this study: Biotin-CD3ε (145-2C11, Biolegend), Biotin-TCRβ (H57-597, Biolegend), Biotin-CD4 (GK1.5, Biolegend), Biotin-CD8α (53-6.7, Biolegend), Biotin-CD19 (6D5, Biolegend), Biotin-CD11c (N418, Biolegend), Biotin-CD11b (M1/70, Biolegend), Biotin-TCRγδ (GL3, Biolegend), Biotin-FcεRI (MAR-1, Biolegend), Biotin-Ly6G/C (RB6-8C5, Biolegend), Biotin-NK1.1 (PK136, Biolegend), Biotin-Ter119 (TER-119, Biolegend), Biotin-EpCAM (G8.8, Biolegend), BV785-CD45 (30-F11, Biolegend), V500-CD90.2 (53-2.1, BD Bioscience), PE-CF594-CD127 (SB/199, BD Bioscience), PE-Cy7-KLRG1 (2F1, Biolegend), BUV395-GATA3 (L50-823, BD Bioscience), BUV496-CD44 (IM7, BD Bioscience), BUV615-TCRβ (H57-597, BD Bioscience), BUV737-CD4 (RM4-5, BD Bioscience), BV421-CD8α (53-6.7, Biolegend), eFlour450-Foxp3 (FJK-16s, Thermo Fisher Scientific), BV480-Siglec-F (E50-2440, BD Bioscience), BV510-IFNγ (XMG1.2, Biolegend), BV570-CD11b (M1/70, Biolegend), BV605-TCRγδ (GL3, Biolegend), BV711-CD11c (N418, Biolegend), BV750-CD90.2 (53-2.1, BD Bioscience), BV786-CD19 (1D3, BD Bioscience), AF488-IL-4 (11B11, Biolegend), PerCP-eFluor710-IL-13 (eBio13A, Thermo Fisher Scientific), PE-IL-5 (TRFK5, Biolegend), PE-CF594-KLRG1 (2F1, BD Bioscience), PE/Fire 640-MHCII (M5/114.15.2, Biolegend), PE-Cy5-CD127 (SB/199, Biolegend), PE/Fire 700-CD45 (30-F11, Biolegend), PE-Cy7-T-bet (4B10, Biolegend), AF700-NK1.1 (PK136, BD Bioscience), APC-Cy7-CD3ε (17A2, BD Bioscience), APC-Fire810-Ly6G/C (RB6-8C5, Biolegend).

Techniques: Staining